George Sourvinos, PhD
Department of Laboratory Medicine –
Medical School, University of Crete
The main interest of our group concerns the control of both HSV-1 and HCMV gene expression during lytic and quiescent infection and the analysis of the virus-host interactions at early stages of the infection. The above questions are approached in a multidisciplinary way, using a variety of techniques of Cell Biology and most important of them, monitoring of live infected cells. Genetically engineered viruses are constructed, expressing autofluorescent proteins (e.g. EGFP) fused to viral proteins, enabling the visualisation of their dynamics. Furthermore, the experimental approaches utilise a variety of molecular genetic techniques such as gene cloning and expression, transient transfection assays and biochemical assays of enzyme activity.
- Regulation of Herpes Simplex Virus type-1 (HSV-1) and Human Cytomegalovirus (HCMV) gene expression during lytic infection and latency.
- The role of Rho GTPases during HCMV infection.
- Signaling pathways activated during HSV-1 lytic infection, latency and reactivation.
- Epigenetic regulation of HCMV infection by the Polycomb Repressive Complexes.
- Activation of inflammatory and epigenetic pathways during HCMV productive infection
via miRNAs of the host.
Our research is funded by the EU FP7 program, the Ministry of Education, Research & Religious Affairs of Hellas and the Special Account for Research of the University of Crete.